Objective:
Today, designing nanofibers with antibacterial properties using electrospinning technology is one of the attractive approaches for wound healing.

Methods & analysis:
We fabricated a nanocomposite from polyethylene oxide (PEO) coated with copper nanoparticles (NPs) and defensin peptide with wound healing and antimicrobial properties in different ratios of CuNPs/defensin (2/0 mg), (1.5/0.5 mg), and (1/1 mg) in the fixed contain polymer (98 mg). Then, the nanofiber properties were investigated by SEM,
tensile, DSC, and BET analysis. Also, the antibacterial properties against S. aureus and E. coli,
antioxidant, and in-vivo wound healing effects and histological analysis of the designed nanocomposites were evaluated in rat models.

 Results:
Our SEM images showed that CuNPs and defensin were properly coated on the PEO
surface. According to the tensile, DSC, and antibacterial analysis results, the most appropriate
feature was related to CuNPs/defensin (1.5/0.5 mg), with maximum elasticity, heat resistance,
and antibacterial activity. Furthermore, the designed nanocomposites showed the best performance as a wound closure agent by increasing dermis and epidermis volume density, stimulating
fibroblast cells and collagen fiber production, and improving skin vessels.

Conclusion:
According to our results, PEO nanofibers loaded with CuNPs and defensin have the
best potential for wound healing, and they can be used as antibacterial materials in the textile,
drug, and medical industries.

Dr. Farhad Moradi (Specialist in Medical laboratory Sciences (PhD, MSc & BSc) & Doctor of Philosophy in Medical and clinical Bacteriology (Ph.D.)) has completed his PhD from Department of Bacteriology & Virology, School of medicine, Shiraz University of Medical Sciences, Shiraz, Iran.