Background: The spread of antibiotic-resistant bacteria has become a pressing global health, endangering the effectiveness of our most potent antibiotics. This study investigates the antibacterial efficacy of the Adam series compounds against multidrug-resistant (MDR) clinical isolates.

Methods: Gram positive and negative bacteria used in this investigation. HBEC-5i cells were obtained from the Adexbio and cultured in RPMI. At around 80 to 90 percent cell confluence. The cell cytotoxicity potential of Adam series was performed using Lactate dehydrogenase (LDH) assays procedure against HBEC-5i cells.

Results: Adam series compounds exhibited bactericidal effects with exceptions noted for Adam 11S and Adam 16S against S. pneumoniae. Adam 10S displayed the highest antibacterial activity, eliminating 70% of bacteria, while Adam 17S and Adam 11S eliminated 50% and 35% of S. pneumoniae, respectively. Against S. pyogenes, all compounds demonstrated bactericidal activity, with Adam 1S exhibiting complete bacterial eradication and Adam 16S displaying an 84% killing effect. Similarly, antibacterial properties were observed against B. cereus, with Adam 1S achieving 100% bacterial elimination. Remarkable bactericidal effects were also noted against S. marcescens, E. coli K1, and S. enterica. MIC50 analysis revealed potent antibacterial activity of the compounds against S. marcescens and S. pyogenes. Additionally, lactate dehydrogenase assays indicated minimal cytotoxicity of the compounds on human microvascular endothelial cells, with Adam 10S, Adam 11S, Adam 16S, and Adam 17S demonstrating less than 20% cytotoxicity, while Adam 1S exhibited 32% cytotoxic effects against human cells.

Conclusion: Adam series compounds have antibacterial efficacy and low cytotoxicity profile, suggesting their potential for further development as therapeutic agents against drug-resistant bacterial infections

Balsam Qubais is Associate Professor in in Clinical Microbiology in Collage of Medicine, Sharjah, UAE, Balsam has completed her BSC in Biology in 1999 and her MSc Degree in Bacteriology in 2001 from University of Mosul, she got her PhD Degree, in Medical Parasitology from Tikrit University in 2006.
She joined the Clinical Sciences Department in Collage of Medicine, UOS, since September 2017.
Balsam has been teaching in both the undergraduate and postgraduate programs. She taught many courses in the Human Biology and Clinical Microbiology, she has published more than 45 research articles in peer reviewed international journals, most of them in Q1 and Q2 journals, she is a reviewer for many international journals. She has also participated in many national and international conferences. She published a book chapter titled “Polymerase Chain Reaction: Types and Applications “.