Alzheimer’s disease (AD) is the most common neurodegenerative disorder, which

is characterized by a progressive loss of neuronal cells in different brain’s regions

that lead to a functional cognitive impairment. Neurofibrillary tangle (NFT) is a

primary biomarker of AD, which is formed by an abnormal accumulation of hyper

phosphorylated Tau protein inside the neuronal cells. Effective treatment for

neurodegenerative diseases (NDs) presents a clinical challenge. Natural products

showed a promising role in treatment of neurodegenerative diseases (NDs).

Geniposide is a natural extract from the fruit of Gardenia Jasminoides, which is

known to have significant biological activities such as anti-inflammation, anti-

oxidation, and anti-diabetic effects. In this project we aim to assess the ability of

using Geniposide as a neuroprotective agent, and to identify the efficiency of

Geniposide in preventing Tauopathy in human neuroblastoma cell lines. Firstly,

300 μM zinc sulfate (for 4 hours) was used to establish an in vitro Tauopathy

model using SH-SY5Y cells. Then, we examined the neuroprotective ability of

Geniposide using different concentrations (10-25-50-75-100 μM), all

concentrations showed significant results with MTT assay. Next, we are studying

the efficacy of Geniposide in the prevention of hyper-phosphorylation of Tau

protein. Geniposide decreases the hyper-phosphorylated Tau significantly. We are

also investigating the role of different protein kinases such as (PI3K, AKT, mTOR,

GSK-3β, and p-Tau) to reveal the molecular pathway by which Geniposide works.

Therefore, Geniposide is showing a promising therapeutic strategy for Alzheimer’s

disease.

Miss. Inas Birekdar is a professional based in Cairo, Egypt, currently affiliated with The American University in Cairo. With a strong commitment to academic and professional excellence, Miss Birekdar is actively engaged in her role within the institution