Objective: The present study was carried out to evaluate the antitumor and antioxidant activities of the methanol extract of Cordia dichotoma (MECD) against EAC in Swiss albino mice. Materials and Methods: The present study evaluated the anticancer effect of the methanolic extract of Cordia dichotoma (MECD) bark against Ehrlich Ascites carcinoma (EAC) cells induced in albino mice and against human cancer cell lines (malondialdehyde-MB-231 andMCF-7cells).Results and Discussion: There was significant fall in the red blood cell count and hemoglobin (Hb) content, and a significant increase in white blood cell (WBC) count in the EAC control mice as compared to normal control mice. Treatment with MECD (500 mg/kg, b.w., p.o.) or 5-Fluorouracil (20 mg/kg b.w., i.p.)in EAC-cell bearing mice caused a significant (P < 0.01) increase in Hb levels while a significant (P < 0.01) decrease in WBC levels compared to EAC control rats. Further more, increase in the concentration of MECD dose-dependently increased the percent cytotoxicity and decreased the cell viability in both cell line types. The results with MECD were comparable to the tamoxifen. The maximum gain of body weight was observed in the EAC control group. In case of MECD and 5-Fluorouracil treated groups, the body weight was significantly (P < 0.01) reduced. The tumor volume and tumor weight were found to be significantly (P<0.05orP<0.01) decreased in MECD treated animals at the doses 250 and 500mg/kg and 5-Fluorouracil (20mg/kg) when compared with EAC control animals. With MECD treatment, the survival of EAC bearing mice significantly (P < 0.01) increased as compared to EAC bearing control group. In treated group, mean survival time (MST) was significantly increased to 29.0±1.98 (%ILS=69.04), 34.12±1.84 (%ILS=81.25), and 36.87±1.67 (%ILS=87.79), respectively, whencompared to EAC control group. Conclusion: The results of the current study proposed that the antitumor activity of MECD can be inferred from the increased life span of EAC bearing mice which is due to its antioxidant activity.

Dr. Nazim Hussain joined the Center for Applied Molecular Biology (CAMB) PU, Lahore in 2006 as a forensic scientist and after preliminary training at CAMB went to Indianapolis USA for hands-on training in evidence examination, serology procedures, DNA extraction, Real-time PCR and, DNA Short Tandem Repeat (STR) analysis using ABI 310 Genetic Analyzer. He solved more than 300 criminal cases including parentage, theft, rape, murder, sodomy, robbery, mass disaster, and dead body identification at CAMB. Meanwhile, more than a dozen symposium, and hands-on training workshops were arranged for Medico legal Doctors, Judiciary personals, and police officials. Later he joined the department of biochemistry and molecular biology at Xian Jiaotong University Xian China for his Doctoral programme. During his Ph.D. at Xian Jiaotong medical university, he explored a novel miRNA involved in the regulation of rheumatoid arthritis and proposed its therapeutic importance for the treatment of RA. He is foreign PhD degree holder along with 48.5 impact factor. He has also been recognized as HEC approved PhD supervisor and had published more than 16 publications.
Currently, along with teaching/supervising M-Phil and Ph.D students, also providing molecular diagnosis of Covid-19, HCV, and HBV at molecular diagnostic laboratory CAMB.